Skip to main content
. 2015 Apr 10;10(4):e0123422. doi: 10.1371/journal.pone.0123422

Fig 2. Phosphate-dependent colonization of cmp2 and WT.

Fig 2

(A) Percent root length colonization of cpm2 and wild type roots with Rhizophagus irregularis at four (left) and six (right) weeks post inoculation (wpi) after fertilization with 25μM or 250μM phosphate. Root length colonization was determined by a modified gridline intersect method. Note the difference in scale between four and six wpi. Int hyphae, intraradical hyphae. Means and standard errors of six biological replicates are shown. Each replicate was represented by 20 root pieces of 2 cm length. Significant differences (ANOVA, posthoc Tukey; p≤0.05, n = 48) are indicated by different letter for comparisons within the same category (i. e. fungal structure). F(total)7,36 = 44.188, F(hyphopodia)7,36 = 21.993, F(int. hyphae)7,36 = 24.046, F(arbuscules)7,40 = 30.634, F(vesicles)7,40 = 28.588. B) Expression of arbuscule marker genes AM14 and PT11 at four and six wpi in cmp2 (light grey) and wild-type (dark grey) roots colonized by Rhizophagus irregularis and fertilized with either 25 μM (filled bars) or 250 μM (hashed bars) phosphate. Different letters indicate values that were significantly different (p≤0.05, n = 24) as determined by an ANOVA with posthoc test Tukey (F(AM14)7,16 = 1.676; F(PT11)7,16 = 21.499).