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. 2015 Feb 3;3(2):e12286. doi: 10.14814/phy2.12286

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Cell-to-cell transfer of siRNA. (A) Schematic in the upper panel illustrates experimental conditions. Middle panel: cell 1- HeLaCx43 cell transfected with siRNA Cy-3 (red), cell 2- recipient cell transfected with mHCN2 gene and eGFP (green). After 24 h in coculture red-labeled siRNA Cy-3 can be detected in the recipient cell (green) of the pair (lower panel). Scale bar, 10 μm. (B) I_m elicited in cell 2 (green) showed inhibited HCN2 currents. (C) Voltage steps (10 mV, 50 ms) applied to cell 1- and cell 2-induced currents in the cell pair above showing gap junction coupling of g_j = 22 nS. (D) Averages of current densities measured from single control cells (green bar: 71.9 ± 4.7 pA/pF, n = 54) and cells from the pair with siRNA-transfected cells (pink bar: 35.7 ± 4.1 pA/pF, n = 51), P < 0.001.