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. 2015 Feb 3;3(2):e12286. doi: 10.14814/phy2.12286

Figure 3.

Figure 3

Extracellular delivery of siRNA. (A) Upper panel: schematic of experimental conditions for HEK293 cells. Middle panel: coculture of HEK293 cells expressing mHCN2 and eGFP with siRNA Cy-3 preloaded HEK293 cells for 48 h. eGFP cells not in contact with the siRNA Cy-3 cells contain siRNA Cy-3 with vesicular compartments. Scale bar, 10 μm. Lower panel: coculture of HeLaCx43 cells expressing mHCN2 and eGFP with siRNA Cy-3 preloaded HeLaCx43 cells for 24 h. eGFP cells not in contact with the siRNA Cy-3 cells contain siRNA Cy-3 with vesicular compartments. Scale bar, 20 μm. (B) Currents from single HEK293 mHCN2-expressing cells (green traces) and HCN2 currents from those single cells containing siRNA Cy-3 vesicular compartments (red traces). (C) Histogram for all HEK293 cells data collected: control, HCN2-expressing cells (green bar: 62.1 ± 6.5 pA/pF, n = 28) and HCN2-expressing cells containing siRNA Cy-3 vesicular compartments (pink bar: 9.1 ± 2.5pA/pF, n = 25), P < 0.001. (D) Average of current densities measured from single control HeLaCx43/HCN2 cells (green bar: 86.0 ± 7.4 pA/pF, n = 18) and from single HeLaCx43/HCN2 cells containing siRNA Cy-3 vesicular compartments (pink bar: 30.7 ± 5.3 pA/pF, n = 16), P < 0.001.