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. 2015 Feb 3;3(2):e12286. doi: 10.14814/phy2.12286

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Gap junction-mediated transfer of siRNA in the presence of Bafilomycin. (A) Upper panel: schematic of experimental conditions. (B) In the presence of Bafilomyocin A1 (24 h) no significant siRNA Cy-3 transfer is seen in HeLaCx43 cells. Scale bar, 20 μm. (C) Middle and lower panels: HeLaCx43 cell pair incubated with Bafilomyocin A1 for 24 h. In this case siRNA Cy-3 is observed in the recipient (eGFP) cell. Scale bar, 10 μmol/L. (D) I_m elicited in cell 2 (green cell) showed inhibited HCN2 currents. (E) Averages of current densities measured from single control cells (green bar: 71.9 ± 4.7 pA/pF, n = 54) and cells from the pair with siRNA-transfected cells (pink bar: 21.8 ± 8.2 pA/pF, n = 9) incubated with Bafilomycin A, P < 0.001. (F) Summary of HCN2 currents recorded from HEK/HCN2 cells incubated with Bafilomycin A1 for 24 h (gray bar: 59.5 ± 5.8 pA/pF, n = 66 and control cells (green bar: 63.1 ± 5.2 pA/pF, n = 62), P = 0.474.