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. 2015 Feb 3;3(2):e12286. doi: 10.14814/phy2.12286

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Morpholino transfer. (A) HEK293 cell pair where cell 2 is expressing eGFP and HCN2 and the cell 1 has been transfected with a red-tagged morpholino targeting HCN2. (B) Typical I_m recording from a recipient cell of a pair, indicating inhibited HCN2 currents (g_j = 14 nS). Scale bar, 10 μm. (C) Summary plot of HCN2 currents recorded from HEK293/HCN2 cells. Green bar: single control cells (74.3 ± 6.3 pA/pF, n = 43); pink bar: single cells transfected with morpholino (26.2 ± 2.8 pA/pF, n = 50); striped pink bar: recipient cells of a pair (17.8 ± 5.9 pA/pF, n = 5); P = 0.473. (D) HEK293/HCN2 cells cultured for 24 h in conditioned media obtained from morpholino-transfected HEK293 cells. No significant morpholino transfer via the extracellular space is observed. Scale bar, 20 μm.