Figure 1.

Effect of focal application of mechanical stress on [Ca²⁺]_s. (A) Expression of members of the TRPV family in HT1080 cells. mRNA expression of the members of the TRPV2 family was measured by RT-PCR. (B) Reversibility of the plasma membrane. Mechanical stress was applied using a glass pipette (a, b). The plasma membrane recovered 1 min after the removal of the pipette (c, d). a, c: bright field, b, d: plasma membrane was made visible by transfection of PM-GFP. (C) Effect of focal application of mechanical stress on the localization of Zyxin. Focal mechanical stress was applied to the cell for 5 min as indicated by the arrow (b). Localization of zyxin was determined by monitoring fluorescence of GFP-zyxin. a: before application of the mechanical stress. (D) Changes in [Ca²⁺]_s in a Cell Receiving Focal Mechanical Stress. Focal mechanical stress was applied as indicated by the arrow and changes in [Ca²⁺]_s were monitored using pm-Cameleon-nano. Warm color indicates elevation of [Ca²⁺]_s. The results are representative of those obtained in five experiments. a: 0 min, b: 0.5 min, c: 1 min, d: 2 min, e: 5 min, f: 10 min.