Figure 2.

Effect of focal administration of mechanical stress on [Ca²⁺]_s. Focal mechanical stress was administered using a pipette (A) and changes in [Ca²⁺]_s at B, C and D were monitored by using pm-Cameleon-nano. E: Changes in [Ca²⁺]_s at B in the absence of extracellular calcium. Results are representative of five experiments. (F) Effect of removal of extracellular calcium on elevation of [Ca²⁺]_s. Experiments were carried out as shown in Fig.2B and 2E and area under the curve (AUC) was calculated. Values are the mean ± SE for five experiments. *P < 0.01 versus 1.3 mmol/L calcium.