Figure 3.

Role of TRPV2, PI 3-kinase and Rac in elevation of [Ca²⁺]_s induced by focal mechanical stress. (A) Mechanical stress was applied focally as shown in Figure2 and changes in [Ca²⁺]_s beneath the pipette were monitored in the presence of 10 μmol/L ruthenium red. The result is a representative of three experiments. (B) mRNA expression levels were measured by quantitative RT-PCR in control and TRPV2 knocked down (KD) cells. Values are the mean ± SE for four experiments. (C) Efficiency of Knockdown of TRPV2. Cells were transfected with control vector (a) or shTRPV2 (c). Protein expression of TRPV2 was visualized in b and d. Note that TRPV2 expression was almost completely abolished by shTRPV2 (d). (D) Mechanical stress was applied in a TRPV2 knocked down cell and changes in [Ca²⁺]_s were monitored. The result is a representative of four experiments. (E) Mechanical stress was applied in the presence of 100 μmol/L LY29034. The result is a representative of five experiments. (F) Mechanical stress was applied in cell expressing dominant negative Rac. The result is a representative of three experiments. (G) Cells were treated with or without latrunculin and actin filaments were visualized. (H) Mechanical stressed was applied in cells treated with latrunculin. The result is a representative of three experiments.