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. 2015 Feb 25;3(2):e12310. doi: 10.14814/phy2.12310

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Production of sFlt-1 (Panel A) and ANGII (Panel B) from explants isolated from normal pregnant (NP) and reduced uterine perfusion pressure (RUPP) rats. NP placental explants were cultured for 30 h in normoxic conditions of 6% O₂, 5% CO₂, 89% O₂ whereas RUPP placental explants were maintained at 1% O₂, 5% CO₂, 94% O₂ for 30 h prior to collect of media. Data expressed as mean ± SEM. A P value of 0.05 was considered statistically significant.