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Gene expression stability and pairwise variation of the candidate reference genes for accurate normalization in sublingual gland. (A) In this analysis, Hprt1 and Pole4 were the two most stable genes, followed by Actb. (B) Although using the two most stable genes was sufficient to obtain a V score of 0.14, which is less than the cut-off of 0.15, the downward trend of V values suggested that using the three best references genes would be more optimal (3/4 = 0.12).
