Table 2.
Primers used for the real-time quantitative RT-PCR assays
| Gene symbol | Accession # | Forward primer sequence | Position | Reverse primer sequence | Exon-exon boundary | AE |
|---|---|---|---|---|---|---|
| Actb | NM_007393 | 5′-AGCCTTCCTTCTTGGGTATGG-3′ | 788–862 | 5′-CAACGTCACACTTCATGATGGA-3′ | Yes | 1.89 |
| B2m | NM_009735 | 5′-TCCAGAAAACCCCTCAAATTCA-3′ | 62–127 | 5′-GTATGTTCGGCTTCCCATTCTC-3′ | Yes | 1.95 |
| Dcpp2 | NM_001039238 | 5′-CCGGGTATTTCTTCAGGCTAGA-3′ | 144–209 | 5′-CCGTCCTCGTTGTCATTATAGTTG-3′ | Yes | 1.99 |
| Gapdh | AK144690 | 5′-TGTGTCCGTCGTGGATCTGA-3′ | 711–787 | 5′-CCTGCTTCACCACCTTCTTGA-3′ | Yes | 1.93 |
| Has1 | NM_008215 | 5′-TCAGGGAGTGGGATTGTAGGA-3′ | 1980–2040 | 5′-AAATAGCAACAGGGAGAAAATGGA-3′ | No | 1.86 |
| Hprtl | NM_013556 | 5′-TGACACTGGCAAAACAATGCA-3′ | 711–774 | 5′-GGTCCTTTTCACCAGCAAGCT-3′ | No | 1.84 |
| Pole4 | NM_025882 | 5′-CGGGACAGGAAGCCATCTT-3′ | 269–348 | 5′-AGCAGTAGGCATCTTTTGCGATA-3′ | Yes | 1.88 |
| Ppia | AK151583 | 5′-AGGGTTCCTCCTTTCACAGAATTA-3′ | 146–219 | 5′-AGTGCCATTATGGCGTGTAAA-3′ | Yes | 1.99 |
| Ppib | NM_011149 | 5′-GGTGGAGAGCACCAAGACAGA-3′ | 543–609 | 5′-GCCGGAGTCGACAATGATG-3′ | No | 1.84 |
| Prrt1 | NM_030890 | 5′-GTCCGCCACACGACTACATG-3′ | 653–789 | 5′-GATCTCGGCAGACACCAAATC-3′ | Yes | 1.86 |
| Rpl27a | NM_011975 | 5′-AAAGCCGTCATCGTGAAGAAC-3′ | 64–164 | 5′-GCTGTCACTTTCCGGGGATAG-3′ | Yes | 1.93 |
| Sdha | NM_023281 | 5′-CGGCTTTCACTTCTCTGTTGGT-3′ | 93–168 | 5′-TGGGTATTGAGTAGAAATTGCATCTG-3′ | Yes | 1.89 |
Primer sequences and positions for the reference sequence are given. When possible, primers were designed to span exon–exon junctions to avoid amplification of contaminating genomic DNA. Real-time qRT-PCR amplification efficiencies (AE) were calculated from the actual PCR runs.