Table 6.
Gene expression (ΔCT) in salivary glands of B6.CAST-17.1 homozygous subcongenic compared to wild-type mice, when fed high- or low-fat diet
| Gene symbol | Wild-type | Subcongenic | Wild-type | Subcongenic | ||
|---|---|---|---|---|---|---|
| Tissue | High-fat1 | P-value | Low-fat1 | P-value | ||
| Dcpp2 | ||||||
| Sublingual | −11.31 ± 0.14 | −9.29 ± 0.69 | <0.05 | −10.88 ± 0.47 | −8.09 ± 0.53 | <0.05 |
| Prrt1 | ||||||
| Sublingual | 7.24 ± 0.42 | 6.52 ± 0.33 | ns | 7.18 ± 0.56 | 7.16 ± 0.86 | ns |
| Submandibular | 7.67 ± 0.62 | 7.70 ± 0.35 | ns | 7.95 ± 0.93 | 7.84 ± 0.77 | ns |
| Has1 | ||||||
| Sublingual | 7.37 ± 0.46 | 6.24 ± 1.03 | ns | 7.21 ± 0.61 | 7.66 ± 0.45 | ns |
| Submandibular | 5.83 ± 0.91 | 7.09 ± 0.73 | <0.01 | 4.93 ± 0.81 | 6.77 ± 0.71 | <0.001 |
1
High-fat, D12331; low-fat D12329 (Research Diets, Inc.). The qRT-PCR data were normalized using multiple reference genes (Vandesompele et al. 2002). Genotype comparisons were performed using individual ΔCT values. Values are mean ± SD for 5–11 animals per group. P-values were obtained using the Student's t-test with Bonferroni correction, ns, nonsignificant.