Figure 5.

Treatment with CL 316,243 increases select mitochondrial proteins in white adipose tissue in an IL-6-dependent manner. WT and whole-body IL-6^−/− mice were injected with CL (1.0 mg/kg bw) or an equivalent volume of sterile saline once per day for five consecutive days and changes in UCP-1 mRNA expression (A, C) and mitochondrial proteins and AMPK (B, D) determined in epididymal adipose tissue (eWAT) (A, B) and inguinal adipose tissue (iWAT) (C, D). Data are presented as mean + SEM for seven samples/group. Representative blots are shown in E. *Significantly different than saline treated in the same genotype, #Significantly different than CL treated in WT, P < 0.05. CS, citrate synthase; Cyto C, cytochrome C.