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. 2015 Feb 25;3(2):e12315. doi: 10.14814/phy2.12315

Table 3.

Effects of 8 days of pair-feeding or heat stress on gene expression in adipose tissue.

Gene Description Primers, 5′–3′ Trt, ΔΔCt1 SEM Fdiff4 P
PFTN2 HS3 Trt5
ADRBK1 Adrenergic, beta, receptor kinase 1 F:GCGTCATGCAGAAGTACCTG6 −1.17 −0.53 0.28 −1.56 0.13
R:GCTTCAGGCAGAAGTCTCGG7
ATGL (PNPLA2) Adipose triglyceride lipase (Patatin-like phospholipase domain containing 2) F:GTGGCCACGGCCCTGGTTAC −0.43 0.21 0.23 −1.56 0.07
R:CGGTTCTTGGGCCCACTGCA 
ATP5J2 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2 F:AGATGACGTCAGTTGTACCGC 0.69 0.28 0.30 1.33 0.35
R:ATGCCCGAAGGGGTGAAATC
CAPN1 Calpain 1, (mu/L) large subunit F:GAGCTGTTCTCAAACCCCCA −0.95 −0.50 0.22 −1.37 0.16
R:GGGTGTCGTTGAGGGTAAGG
CD14 CD14 molecule F:CCTTGCAAGATCCTTCCGGT 0.10 −0.73 0.46 1.78 0.23
R:TTGGCAGACTTTGGGGGTTT
CIRBP Cold inducible RNA-binding protein F:GAGTCAGGGTGGCAGCTATG 0.70 0.12 0.31 1.49 0.21
R:ACCCTTCTGAGTTGCACTGG
FADS1 Fatty acid desaturase 1 F:CGTGATTGACCGGAAGGTGT −2.26 −2.16 0.34 −1.07 0.83
R:ACAAGGCCCTGGTTGATGTG
FASN Fatty acid synthase F:CTCATCGGCGGTGTGGACAT 0.69 0.90 0.37 −1.16 0.69
R:CATCGTGTTCGCCTGCTTGG
G6PD Glucose-6-phosphate dehydrogenase F:GCGATGCTTTCCATCAGTCG 1.06 1.62 0.36 −1.47 0.29
R:GCGTAGCCCACGATGTATGT
GSK3B Glycogen synthase kinase 3 beta F:CGAGACACACCTGCACTCTT −0.62 −0.18 0.29 −1.36 0.30
R:TGACGCAGAAGCGGTGTTAT
HSF1 Heat-shock transcription factor 1 F:TTCAAGCACAGCAACATGGC −0.51 −0.19 0.26 −1.25 0.40
R:CACGCTGGTCACTTTCCTCT
HSF2 Heat-shock transcription factor 2 F:AGGCCAGGATGACTTGTTGG −0.64 −1.12 0.41 1.39 0.43
R:ACACCTCCTTCCAAAGGGAC
HSP90AA1 Heat-shock protein 90 kDa alpha (cytosolic), class A member 1 F:ATCGCCCAGTTGATGTCGTT 0.21 −0.73 0.41 1.92 0.13
R:TATCGTGAGGGTCCGGTCTT
HSPA2 Heat-shock 70 kDa protein 2 F:TGAGAGTTTCCAGAAGGCGG 0.02 −0.92 0.55 1.92 0.25
R:AAGACGAGCAAGCGACGTTA
HSPA4 Heat-shock 70 kDa protein 4 F:CGCTTCGCAGTGTTTTGGAA 0.05 −1.23 0.48 2.43 0.08
R:TGCACAGCCTCGAGTAACAG
HSPB8 Heat-shock 22 kDa protein 8 F:GATGGCTACGTGGAGGTGTC −0.09 −1.42 0.53 2.51 0.10
R:GGGGAAAGCGAGGCAAATAC
HSPCB Heat-shock protein 90 kDa alpha (cytosolic), class B member 1 F:CCGTTCTCTTGAGTCACCCC 0.19 0.01 0.27 1.13 0.66
R:GAGACATGAGCTGGGCGATT
HSPE1 Heat-shock 10 kDa protein 1 (chaperonin 10) F:AGCTGTTGGATCAGGCTCTAAA 1.76 0.40 0.52 2.57 0.09
R:TGTGATGCCATTAGACAGTGAC
IDH2 Isocitrate dehydrogenase 2 (NADP+), mitochondrial F:CGACCAGAGGATCAAGGTGG 0.06 0.18 0.16 −1.09 0.59
R:GGGAGCCCCAGGTCAAAATA
INSR Insulin receptor F:CAGCCTGCGAGAGCGGATCG −0.97 −0.42 0.19 −1.46 0.07
R:TGAGAATAACCCCGGCCGCC
LDHA Lactate dehydrogenase A F:AAGGAACACTGGAAAGCGG 1.10 0.56 0.47 1.45 0.44
R:CATGGTGGAAATCGGATGCAC
MAPK14 Mitogen-activated protein kinase 14 F:CAGGGGCTGAGCTTTTGAAG −0.07 −0.39 0.24 1.25 0.35
R:GCAAGTCAACAGCCAAGGGA
MDH2 Malate dehydrogenase 2, NAD (mitochondrial) F:TTCTTGCTGCCAGCTCGTTT −0.05 0.46 0.41 −1.42 0.39
R:AGCACAGCTACCTTGGCATT
MIF Macrophage migration inhibitory factor (glycosylation-inhibiting factor) F:ATCAGCCCGGACAGGATCTA 0.02 0.70 0.27 −1.60 0.10
R:GCCGAGAGCAAAGGAGTCTT
MKNK2 MAP kinase interacting serine/threonine kinase 2 F:AAGAAGAAGAGGTGCCGAGC −0.65 −0.10 0.25 −1.46 0.15
R:CACCTCCCGGAAAACCCTAC
NDUFB7 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18 kDa F:CGCATGAAGGAGTTTGAGCG −0.03 0.53 0.23 −1.47 0.12
R:GGGGCCTGGAGGCTTTTATT
NDUFS7 NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20 kDa F:TGCCAGAGCCTCGTTATGTT 0.13 0.42 0.23 −1.22 0.40
R:CAGTCTCTTCTCCCGCTTGA
NDUFV1 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51 kDa F:CGCGAGTATCTGTGCGTTTC −0.17 0.32 0.22 −1.40 0.13
R:TTCAGTCTCCAGTCATGGCG
PC Pyruvate carboxylase F:AAGCCCTGGCCATAAGTGAC 0.30 0.48 0.24 −1.13 0.60
R:CCCAATCTGGCCCTTCACAT
PDAP1 PDGFA-associated protein 1 F:GTGGAAGGGCTCATCGACAT 0.61 1.40 0.28 −1.73 0.07
R:GCTCTGTCTTCCCCGCTAAA
PDCD7 Programmed cell death 7 F:TCTTACAGCCTTTCCGGCAG −1.17 −1.22 0.37 1.04 0.92
R:TTAATGGCGGTTGCCCAGAT
PEMT Phosphatidylethanolamine N-methyltransferase F:ATGGAGCGCGTGTTTGACTA 0.32 0.40 0.27 −1.06 0.84
R:TCCTGGGATCTCGTTCTCGT
PLIN Perilipin F:CAACAAGGGCCTGACTTTGC −1.63 −0.44 0.71 −2.28 0.26
R:ATTGCATACAGACGCCACCA
PRKAG1 Protein kinase, AMP-activated, gamma 1 noncatalytic subunit F:GCATCCTCAAGACACCCCAG −0.59 0.33 0.30 −1.89 0.05
R:GCAGCTCGGACACCATTAGT
PRLR Prolactin receptor F:ACAGTCACCTCCGGGAAAAC −1.15 −0.92 0.63 −1.17 0.79
R:TAGGGCCGCCAGTTTTGTAG
RBM7 RNA-binding motif protein 7 F:AGCAGGTACGAAAGAACGGT −0.13 −0.72 0.31 1.51 0.21
R:GGGACTGAACTGATGGCGAA
UBE2B Ubiquitin-conjugating enzyme E2B F:GGTGACCCACAGTGATTCGG −1.03 −1.48 1.42 1.37 0.81
R:AGGTGGGTCCTCTTGCAATC
UBE2G1 Ubiquitin-conjugating enzyme E2G 1 F:TCGCCCGCTGTGTAAGAAAA 0.92 0.19 0.70 1.66 0.47
R:CAGTTCCAGCCAGTGTTTGC
ZAP70 Zeta-chain (TCR)-associated protein kinase 70 kDa F:CAACTTTGGCTCTGTTCGCC 0.35 1.54 0.57 −2.28 0.19
R:CACGTTGCTGACAGGGATCT
ZSCAN29 Zinc finger and SCAN domain containing 29 F:CCCACAGGAGAAGCTCAGAC −1.13 −0.31 0.26 −1.77 0.05
R:CCCAGTGATCCTGTTCCACC
1

Delta delta Ct.

2

Pair-fed thermoneutral.

3

Heat stress.

4

Fold difference: positive/negative values indicate increased/decreased transcript abundance in HS pigs compared to PFTN controls.

5

Treatment.

6

Forward.

7

Reverse.