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. 2015 Feb 25;3(2):e12315. doi: 10.14814/phy2.12315

Table 5.

Effects of 8 days of pair-feeding or heat stress on gene expression in liver.

Gene Description Primers, 5′–3′ Trt, ΔΔCt1 SEM Fdiff4 P
PFTN2 HS3 Trt5
ADRBK1 Adrenergic, beta, receptor kinase 1 F:GCGTCATGCAGAAGTACCTG6 0.18 0.02 0.18 1.12 0.54
R:GCTTCAGGCAGAAGTCTCGG7
ATP5J2 ATP synthase, H+ transporting, mitochondrial Fo complex, subu F2 F:GATGACGTCAGTTGTACCGC 0.08 0.34 0.12 −1.20 0.14
R:AATGCCCGAAGGGGTGAAAT
CAPN1 Calpain 1, (mu/L) large subunit F:TGCACCGAGTAGTTCCACAC 0.52 0.70 0.59 −1.13 0.17
R:AACTCATTGCCTTGGGCAGA
CCRN4L CCR4 carbon catabolite repression 4-like (S. cerevisiae) F:CCCGCTTCCAGAGGGATTTT 0.94 1.49 0.34 −1.46 0.27
R:GGGCTGGTAGGCTAGGATCT
CD14 CD14 molecule F:AACCCCCAAAGTCTGCCAAA −0.32 0.08 0.26 −1.32 0.28
R:AAGGTCCTCAAAGCCTCTGC
CIRBP Cold inducible RNA-binding protein F:GAGTCAGGGTGGCAGCTATG 0.13 0.18 0.13 −1.04 0.81
R:ACCCTTCTGAGTTGCACTGG
FADS2 Fatty acid desaturase 2 F:GCGCAGATGCCTACCTTT 0.48 0.65 0.25 −1.13 0.64
R:CTAAATCAAGGTCGCGGTGG
FASN Fatty acid synthase F:TCATCGGCGGTGTGGACAT 2.56 1.17 0.24 2.62 <0.01
R:CCATCGTGTTCGCCTGCTTG
G6PD Glucose-6-phosphate dehydrogenase F:GCGATGCTTTCCATCAGTCG 1.56 0.99 0.46 1.48 0.39
R:GCGTAGCCCACGATGTATGT
GCK Glucokinase (hexokinase 4) F:CTGCCTTGGAAGCCTGTTGG 2.36 1.82 0.64 1.45 0.56
R:ATCTCCTTCTGCATCCGCCT
HIF1a Hypoxia inducible factor 1, alpha subunit F:ATCTCGGGCACAGATTCGC 1.02 0.42 0.46 1.52 0.37
R:TCCTCACACGCAAATAGCTGA
HSF1 Heat-shock transcription factor 1 F:TTCAAGCACAGCAACATGGC 0.14 0.14 0.20 1.00 1.00
R:CACGCTGGTCACTTTCCTCT
HSF2 Heat-shock transcription factor 2 F:AGGCCAGGATGACTTGTTGG 0.14 −0.07 0.17 1.16 0.40
R:ACACCTCCTTCCAAAGGGAC
HSP90AA1 Heat-shock protein 90 kDa alpha (cytosolic), class A member 1 F:ATCGCCCAGTTGATGTCGTT 0.53 0.38 0.13 1.11 0.45
R:TATCGTGAGGGTCCGGTCTT
HSPA2 Heat-shock 70 kDa protein 2 F:TGAGAGTTTCCAGAAGGCGG −0.46 −0.62 0.52 1.12 0.83
R:AAGACGAGCAAGCGACGTTA
HSPA4 Heat-shock 70 kDa protein 4 F:AAAGATTCCATGGCCGAGCA 0.59 0.26 0.14 1.26 0.12
R:GCCGTCTCCTTCAGTTTGGA
HSPB8 Heat-shock 22 kDa protein 8 F:GATGGCTACGTGGAGGTGTC 0.28 0.39 0.18 −1.08 0.69
R:GGGGAAAGCGAGGCAAATAC
HSPCB Heat-shock protein 90 kDa alpha (cytosolic), class B member 1 F:AGCCGTTCTCTTGAGTCACC −0.10 −0.18 0.24 1.06 0.81
R:TGCCTGGAAGGCAAAAGTCT
HSPE1 Heat-shock 10 kDa protein 1 (chaperonin 10) F:TTGCAAGCAACCGTAGTAGC 1.11 0.41 0.26 1.62 0.08
R:ACAGTGACTTGTTTCAGTCTACG
IDH2 Isocitrate dehydrogenase 2 (NADP+), mitochondrial F:CGACCAGAGGATCAAGGTGG 0.69 0.40 0.30 1.22 0.50
R:GGGAGCCCCAGGTCAAAATA
KCTD6 Potassium channel tetramerization domain containing 6 F:TGGATAATGGAGACTGGGGCT 0.53 0.09 0.16 1.36 0.08
R:AGCATGGAATCCGGGTAACG
LDHA Lactate dehydrogenase A F:TTGTTGGGGTTGGTGCTGTT 0.74 0.76 0.20 −1.01 0.96
R:TGGGGTCCTAAGGAAAAGGC
LIPG Lipase, endothelial F:CTGGTTCTGGTTCAAGCCCT 0.09 0.32 0.31 −1.17 0.60
R:GATCAGACAGTGGTGGCCTT
MAPK14 Mitogen-activated protein kinase 14 F:CAGGGGCTGAGCTTTTGAAG 0.84 0.34 0.29 1.41 0.25
R:GCAAGTCAACAGCCAAGGGA
MDH2 Malate dehydrogenase 2, NAD (mitochondrial) F:TTTCTTGCTGCCAGCTCGTT 0.54 0.71 0.44 −1.13 0.80
R:GCACAGCTACCTTGGCATTG
MIF Macrophage migration inhibitory factor (glycosylation-inhibiting factor) F:ATCAGCCCGGACAGGATCTA 0.67 0.59 0.31 1.06 0.85
R:GCCGAGAGCAAAGGAGTCTT
MKNK2 MAP kinase interacting serine/threonine kinase 2 F:AAGAAGAAGAGGTGCCGAGC 0.73 0.38 0.23 1.27 0.29
R:CACCTCCCGGAAAACCCTAC
NDUFA6 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 6, 14 kDa F:CAGAGCCTTGCATGTCGGTA −0.91 −1.02 0.15 1.08 0.60
R:AGCTAACCAATCCTGGTGGC
NDUFB7 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18 kDa F:CGCATGAAGGAGTTTGAGCG 0.12 0.48 0.12 −1.28 0.05
R:GGGGCCTGGAGGCTTTTATT
NDUFS7 NADH dehydrogenase (ubiquinone) Fe-S protein 7, 20 kDa F:TGCCAGAGCCTCGTTATGTT 0.02 0.39 0.16 −1.29 0.10
R:CAGTCTCTTCTCCCGCTTGA
NDUFV1 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51 kDa F:CGCGAGTATCTGTGCGTTTC 0.10 0.41 0.22 −1.24 0.32
R:TTCAGTCTCCAGTCATGGCG
PC Pyruvate carboxylase F:AAGCCCTGGCCATAAGTGAC −0.38 −0.34 0.20 −1.03 0.87
R:CCCAATCTGGCCCTTCACAT
PDAP1 PDGFA-associated protein 1 F:GTGGAAGGGCTCATCGACAT 1.10 0.90 0.32 1.15 0.66
R:GCTCTGTCTTCCCCGCTAAA
PDK4 Pyruvate dehydrogenase kinase, isozyme 4 F:AAGCCACATTGGCAGCATTG −0.28 −0.46 0.14 1.13 0.37
R:GGTGTTCGACTGTAGCCCTC
PEMT Phosphatidylethanolamine N-methyltransferase F:ATGGAGCGCGTGTTTGACTA −0.57 −0.43 0.17 −1.10 0.56
R:TCCTGGGATCTCGTTCTCGT
POLD4 Polymerase (DNA-directed), delta 4, accessory subunit F:GCTCTGCTGTGAAGTTTGGC 0.13 0.14 0.13 1.00 0.90
R:AGCCTTTGGAAGGGTCATGG
PRKAG1 Protein kinase, AMP-activated, gamma 1 noncatalytic subunit F:GCATCCTCAAGACACCCCAG 0.38 0.05 0.28 1.26 0.43
R:GCAGCTCGGACACCATTAGT
RBM7 RNA-binding motif protein 7 F:AGCAGGTACGAAAGAACGGT 0.33 −0.06 0.13 1.31 0.06
R:GGGACTGAACTGATGGCGAA
SDHC Succinate dehydrogenase complex, subunit. C, 15 kDa F:CCGTGCCCATCTTAGTCCTC 0.59 0.55 0.21 1.03 0.89
R:GGGGAGACAAAGGACGGTTT
SLC16A5 Solute carrier family 16, member 5 F:TCGGCATCTTCTTCACCGAA 0.15 0.14 0.34 1.01 0.98
R:GATGAATCCTGCCGTGAGGT
SLC25A27 Solute carrier family 25, member 27 F:AAGCTCTGGCAAGGAGTGAC −0.02 −0.23 0.19 1.16 0.44
R:CACGAAATCGCAACGGCTTT
UBE2G1 Ubiquitin-conjugating enzyme E2G 1 F:ACTCGCCTGCTAATGTGGAC 0.45 0.16 0.34 1.22 0.56
R:GTGCAGGAAAAACAGTGCCA
ZAP70 Zeta-chain (TCR)-associated protein kinase 70 kDa F:CAACTTTGGCTCTGTTCGCC −0.07 0.04 0.16 −1.08 0.63
R:CACGTTGCTGACAGGGATCT
ZSCAN29 Zinc finger and SCAN domain containing 29 F:CCCACAGGAGAAGCTCAGAC −0.24 −0.43 0.21 1.14 0.54
R:CCCAGTGATCCTGTTCCACC
1

Delta delta Ct.

2

Pair-fed thermoneutral.

3

Heat stress.

4

Fold difference: positive/negative values indicate increased/decreased transcript abundancein HS pigs compared to PFTN controls.

5

Treatment.

6

Forward.

7

Reverse.