Table 3. Primers used in this study.
Primer name | Sequence 5'- 3' | Citation |
---|---|---|
F8 | TTGGCAGTCTCAGnnnnnnnnAGTTTGATCCTGGCTCAG* | [45] |
R357 | GTCTCCGACTCAGnnnnnnnnCTGCTGCCTYCCGTA* | [45] |
Adapter A | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ** |
Adapter B | CCTATCCCCTGTGTGCCTTGGCAGTCTCAG | ** |
*—nnnnnnnn is the barcode
**—standard adapter for 454 Titanium chemistry