Fig. 5.
Active Lauraceae plant extracts protected human bronchial epithelial cells against sodium arsenite-induced toxicity. A–F Cell survival in human bronchial epithelial cells untreated or pretreated with ZK-08 (60 μg/mL), ZK-02 (40 μg/mL), ZK-04 (60 μg/mL), ZK-05 (30 μg/mL), ZK-10 (60 μg/mL), and sulforaphane (3.0 μM) for 8 hours, and then treated with 20 or 40 μM sodium arsenite in the absence or presence of half of the pretreated doses of Lauraceae extracts and sulforaphane (2.0 μM) for 24 hours. G Immunoblot analysis of nuclear factor-erythroid 2-related factor 2 protein level in human bronchial epithelial cells 36 hours after transfection with nuclear factor-erythroid 2-related factor 2-small interfering RNA. H–K Cell survival was performed as described in A–F in human bronchial epithelial cells transfected with nuclear factor-erythroid 2-related factor 2-small interfering RNA. Values shown are the mean ± SD of experiments run in triplicate. * P < 0.05 vs. plant extract-treated group.