. 2015 Apr 10;10(4):e0123198. doi: 10.1371/journal.pone.0123198

Table 3. Linearity and GroPIns concentration in RBL-2H3 and Jurkat T-cell extracts by internal standard calibration and standard addition method.

	RBL-2H3 cells ^a		Jurkat T-cells ^a
	Internal Standard calibration	Standard Addition calibration	Internal Standard calibration	Standard Addition calibration
GroPIns conc. range (ng/ml)	10–160	10–160	10–160	10–160
Slope	0.0244 ±0.0021	0.0359 ±0.0023	0.0244 ±0.0021	0.0237 ±0.0068
Intercept	0.0718 ±0.0165	0.7745 ±0.0450	0.0718 ±0.0165	0.5145 ±0.0356
Coefficient of correlation (r ² )	0.9994 ±0.0003	0.9993 ±0.0001	0.9994 ±0.0003	0.9992 ±0.0007
GroPIns conc. (ng/ml)	22.04 ±2.44	21.56 ±1.25	19.99 ±2.72	21.67 ±1.77

^aAnalyses were performed in triplicate (n = 5). Values are expressed as mean ± SD.