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. 2015 Apr 10;9(4):e0003663. doi: 10.1371/journal.pntd.0003663

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© 2015 De Jong et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Growth of S. Typhimurium (14028; A) and S. Typhi (Vivotif, Crucell; B) was assessed for a maximum of 24 hours in the presence of recombinant S100A8/A9 (50 μg/ml; grey bars) or control (white bars). Bacterial growth was dose dependently inhibited by S100A8/A9. Growth arrested, CFSE-labelled heat-inactivated S. Typhimurium bacteria were incubated with peripheral blood neutrophils (C) from wildtype (WT; white) and S100A9 ^-/- mice (grey) (n = 4 per mouse strain) for 0, 15 and 60 minutes respectively after which phagocytosis was quantified (see Methods). Data are expressed, as mean ± SD. **** P<0.0001, determined via non-parametric t tests comparing different dosages per time point.