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. Author manuscript; available in PMC: 2015 Oct 9.
Published in final edited form as: Nature. 2015 Apr 1;520(7546):186–191. doi: 10.1038/nature14299

Extended Data Figure 4. Optimization of SaCas9 sgRNA scaffold in mammalian cells.

Extended Data Figure 4

a, Schematic of the Staphylococcus aureus subspecies aureus CRISPR locus. b, Schematic of SaCas9 sgRNA with 21-nt guide, crRNA repeat (gray), tetraloop (black) and tracrRNA (red). The number of crRNA repeat to tracrRNA anti-repeat base-pairing is indicated above the gray boxes. SaCas9 cleaves targets with varying repeat:anti-repeat lengths in c, HEK 293FT and d, Hepa1-6 cell lines. (n=3, error bars show S.E.M.)