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. Author manuscript; available in PMC: 2016 Apr 8.
Published in final edited form as: Cell Host Microbe. 2015 Mar 26;17(4):466–477. doi: 10.1016/j.chom.2015.02.010

Figure 4. IL-1β production by direct activation of RNase L is dependent on both NLRP3 and ASC.

Figure 4

BMDC were primed with Pam3Csk4 (200 ng/ml) for 16 hrs and mock transfected or transfected with (2′-5′)p3A3 for additional 8 hrs as indicated. (A) rRNA cleavage in response to (2′-5′)p3A3 transfection was monitored in RNA chips. (B,C) IL-1β and cleaved caspase-1 was measured in cell supernatants (Sup), whereas proIL-1β, procaspase-1 and β-actin were measured in cell lysates (Lys). Upper panels, ELISAs; lower panels, immunoblots. Error bars, SD; ***; p<0.001 by two-tailed Student’s t tests.