Effect of miR-217 on Wnt5a expression in human renal proximal tubule cells (RPTCs) from subjects carrying D2R-WT or D2R-SNPs before and after treatment with miR-217 mimic or inhibitor.
A-B. mRNA (A) and protein (B) expression of Wnt5a in RPTCs. mRNA expression was quantified by qRT-PCR, protein expression was measured by immunoblotting, GAPDH mRNA or protein was used for normalization of the data, n=4-5/group. *P<0.05 vs corresponding controls; ANOVA followed by Holm-Sidak test.