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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Hypertension. 2015 Mar 23;65(5):1118–1125. doi: 10.1161/HYPERTENSIONAHA.114.05096

Figure 5.

Figure 5

Effect of Wnt5-Ror2 pathway onTGFβ1 in human renal proximal tubule cells (RPTCs) from subjects carrying D2R-WT or D2R SNPs.

A. mRNA expressions of TGFβ1 and Ror2 and protein expression of TGFβ1 in RPTC-D2R-WT treated with Wnt5a or Ror2 siRNA or the combination were quantified as above. GAPDH mRNA or protein was used for normalization of the data, n=4-5/group. *P<0.05 vs treatment with vehicle and non-silencing siRNA. ANOVA followed by Holm-Sidak test.

B. mRNA expressions of TGFβ1 and Ror2 and protein expression of TGFβ1 in RPTC-D2R-SNPs treated with Ror2 siRNA were quantified as above. GAPDH mRNA or protein was used for normalization of the data, n=5/group. *P<0.05 vs non-silencing siRNA. t-test.