FIG 3.

Expression levels of S. ambofaciens ATCC 23877 genes controlling the synthesis of desferrioxamine B and E and coelichelin as measured by RT-qPCR. The desC gene (right) is involved in the synthesis of desferrioxamines, and the samR0552 gene (left) is involved in coelichelin biosynthesis. Total RNAs were extracted after 24, 30, 48, and 54 h of growth on 26A agar medium at 26.5°C. The expression levels are shown as the transcript levels of the target genes relative to the transcript level of the housekeeping gene hrdB, which is stable under the tested growth conditions. The data are expressed as the mean value of three biological replicates. The error bars denote standard errors. For each transcript, values with the same letter are not significantly different according to a one-factor ANOVA (P > 0.01).