TABLE 2.
Primer pairs used in qPCR analyses or to determine transposon insertion sites in the P. fluorescens BBc6R8 genome
| Name | Sequence (5′–3′) | Studied gene |
|---|---|---|
| hrdB-F | CGCGGCATGCTCTTCCT | hrdB |
| hrdB-R | AGGTGGCGTACGTGGAGAAC | |
| selR-F | CGACCCGGAGCAGTACAA | selR |
| selR-R | GCAGCAGATGCAGTGGTAGA | |
| pvdD-F | TTGAGCGTGAGGACATTCTG | pvdD |
| pvdD-R | TCGAACCCTTGCAGGTAATC | |
| pvdE-F | ACGTGGGGTGATCAATGAAT | pvdE |
| pvdE-R | TTTTGCCCCACATAATTGGT | |
| pvdO-F | AAGAAAACCGGCCATCACTA | pvdO |
| pvdO-R | TGTCCACTCGTAGACGTTGC | |
| pvdS-F | ACCATCACGTCATCGTTCAA | pvdS |
| pvdS-R | TTCTCCAGCGTCGAAAAGTT | |
| pvdQ-F | TGCGTTTCTACGAGATGCAC | pvdQ |
| pvdQ-R | AAATAGCGAGTCGGGTCCTT | |
| phcf-F | ACGGCTACCAACAAATCCTG | phcF |
| phcf-R | CACCAGCAGATCCACTGAGA | |
| desC-F | ACTGACCGGGCTGTACGA | desC |
| desC-R | CTTCTCCGGCTTCTGGATCT | |
| sam0552-F | CTTCGTCCTGCAGAACTTCC | sam0552 |
| sam0552-R | AGTACGCGCAGGTAGTCGTC | |
| foxI-F | ACGTGCGTTGATTCTGGAAT | foxI |
| foxI-R | GCACATAACAGTGATAAAGC | |
| foxR-F | CGGCAGGCATTGAAGGTATT | foxR |
| foxR-R | CGCTATCGGTATTGAGCTGC | |
| foxA-F | GCAGACAACGTGATCGAGAA | foxA |
| foxA-R | CACACCCTTCAGTCCAACCT | |
| pepSY-F | GTTTCTGATGATGGCGGGAC | pepSY |
| pepSY-R | ACTTGCAGTTTGGGATGCTG | |
| fur-F | CCAGTTTGAAGCAGCAGGAC | fur |
| fur-R | TCCACCAAATCGAATCCATGC | |
| EZR1a | ATGCGCTCCATCAAGAAGAG | |
| EZL2a | TCCAGCTCGACCAGGATG | |
| gfptns2b | ATCACCTTCACCCTCTCCAC | |
| nCEKG2Ac | GGCCACGCGTCGACTAGTACNNNNNNNNNNAGAG | |
| nCEKG2Bc | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC | |
| nCEKG2Cc | GGCCACGCGTCGACTAGTACNNNNNNNNNNATAT | |
| CEKG4d | GGCCACGCGTCGACTAGTAC |
a
Tn5062-specific primer.
b
For first- and second-round PCR of transposon mutants.
c
For first-round PCR of transposon mutants.
d
For second-round PCR of transposon mutants.