TABLE 1.
Bacterial strains used in this work
| Strain | Genotype and/or description | Source or reference |
|---|---|---|
| Escherichia coli Silver | F− deoR endA1 recA1 relA1 gyrA96 hsdR17(rK− mK+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 ϕ80lacZΔM15 λ− | Bioline |
| Desulfovibrio desulfuricans | 11 | |
| ND132 | Wild type | |
| JWN1001 | ND132 (ΔhgcAB)1::(Pnpt-npt-upp); Kmr | 12 |
| JWN1005 | JWN1001 hgcA(1–498) hgcB+-aadAa; Kms Spr; truncation of HgcA after amino acid 166 | This study |
| JWN1006 | JWN1001 hgcA(C93T) hgcB+-aadA; Kms Spr | This study |
| JWN1016 | JWN1001 hgcA(C93A) hgcB+-aadA; Kms Spr | This study |
| JWN1017 | JWN1001 hgcA(W92A) hgcB+-aadA; Kms Spr | This study |
| JWN1018 | JWN1001 hgcA(V91A) hgcB+-aadA; Kms Spr | This study |
| JWN1019 | JWN1001 hgcA(N90A) hgcB+-aadA; Kms Spr | This study |
| JWN1020 | JWN1001 hgcA(I89A) hgcB+-aadA; Kms Spr | This study |
| JWN1021 | JWN1001 hgcA(G88A) hgcB+-aadA; Kms Spr | This study |
| JWN1022 | JWN1001 hgcA(R87A) hgcB+-aadA; Kms Spr | This study |
| JWN1024 | JWN1001 hgcA(K97A) hgcB+-aadA; Kms Spr | This study |
| JWN1025 | JWN1001 hgcA(G98A) hgcB+-aadA; Kms Spr | This study |
| JWN1026 | JWN1001 hgcA(L99A) hgcB+-aadA; Kms Spr | This study |
| JWN1027 | JWN1001 hgcA(F100A) hgcB+-aadA; Kms Spr | This study |
| JWN1028 | JWN1001 hgcA(T101A) hgcB+-aadA; Kms Spr | This study |
| JWN1029 | JWN1001 hgcA(A94S) hgcB+-aadA; Kms Spr | This study |
| JWN1030 | JWN1001 hgcA(A95S) hgcB+-aadA; Kms Spr | This study |
| JWN1031 | JWN1001 hgcA(E312A) hgcB+-aadA; Kms Spr | This study |
| JWN1045 | JWN1001 hgcA(C93H) hgcB+-aadA; Kms Spr | This study |
| JWN1064 | JWN1001 hgcA(N90P) hgcB+-aadA; Kms Spr | This study |
| JWN1065 | JWN1001 hgcA(V91P) hgcB+-aadA; Kms Spr | This study |
| JWN1072 | JWN1001 hgcA(A94P) hgcB+-aadA; Kms Spr | This study |
| JWN1067 | JWN1001 hgcA(A95P) hgcB+-aadA; Kms Spr | This study |
| JWN1068 | JWN1001 hgcA(W92P) hgcB+-aadA; Kms Spr | This study |
| JWN1069 | JWN1001 hgcA(W92F) hgcB+-aadA; Kms Spr | This study |
| JWN1066 | JWN1001 hgcA(Q187 stop) hgcB+-aadA; Kms Spr; HgcA with Glu187 mutated to TAA stop codon | This study |
| JWN1073 | JWN1001 hgcA(G98P) hgcB+-aadA; Kms Spr | This study |
| JWN1013 | JWN1001 hgcA+ hgcB(C95A)-aadA; Kms Spr | This study |
| JWN1014 | JWN1001 hgcA+ hgcB(C96A)-aadA; Kms Spr | This study |
| JWN1015 | JWN1001 hgcA+ hgcB(C95A/C96A)-aadA; Kms Spr | This study |
| JWN1081 | JWN1001 hgcA+ hgcB(C73A)-aadA; Kms Spr | This study |
a
aadA encodes aminoglycoside 3′-adenyltransferase, which confers spectinomycin resistance.