TABLE 3.

Summary of transcriptome comparison of wild-type S. pneumoniae D39 grown in SM17 (0.5% sialic acid plus M17) to that grown in M17

Category and D39 locus tag	Functiona	Ratiob
Upregulated genes
spd_0091	Hypothetical protein	2.4
spd_0115	Hypothetical protein	2.5
spd_0116	Hypothetical protein	3.0
spd_0373	Hypothetical protein	2.2
spd_1263	ABC transporter, ATP-binding/permease protein	2.2
spd_1264	ABC transporter, ATP-binding protein	2.7
spd_1265	Hypothetical protein	2.9
spd_1267	ABC transporter, ATP-binding protein	3.1
spd_1330	Amino acid ABC transporter, permease protein	5.6
spd_1488	ROK family protein, NanK	4.8
spd_1489	N-Acetylneuraminate lyase, putative	3.8
spd_1490	Hypothetical protein	2.6
spd_1491	Hypothetical protein	2.8
spd_1492	Hypothetical protein	2.6
spd_1493	Sugar ABC transporter, permease protein, NanW	2.7
spd_1494	Sugar ABC transporter, permease protein, NanV	2.9
spd_1495	Sugar ABC transporter, sugar-binding protein, NanU	4.4
spd_1496	PTS, IIBC components, NanP	4.2
spd_1497	N-Acetylmannosamine-6-phosphate 2-epimerase 2, NanE	2.8
spd_1504	Sialidase A precursor, NanA	6.8
spd_1505	Hypothetical protein	2.0
spd_1800	Hypothetical protein	2.1
spd_2012	Alpha-glycerophosphate oxidase	3.2
spd_2013	Glycerol kinase	3.3
Downregulated genes
spd_0334	Oligopeptide ABC transporter, oligopeptide-binding protein AliA	−2.4
spd_0447	Transcriptional regulator, GlnR	−5.5
spd_0448	Glutamine synthetase, GlnA	−6.0
spd_0901	Dihydrodipicolinate synthase	−2.0
spd_1098	Amino acid ABC transporter, GlnP	−4.0
spd_1099	Amino acid ABC transporter, GlnQ	−4.0
spd_1158	NADP-specific glutamate dehydrogenase	−3.5
spd_1514	ABC transporter, ATP-binding protein	−3.3
spd_1515	Hypothetical protein	−3.9
spd_1516	Hypothetical protein	−3.6
spd_1956	Dihydroxy-acid dehydratase	−2.0

^aD39 annotation or TIGR4 annotation (27). PTS, phosphotransferase system.

^bFold increase or decrease in the expression of genes in SM17 compared to M17. Errors in the ratios never exceeded 10% of the given values.