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. 2015 Apr 10;81(9):3235–3242. doi: 10.1128/AEM.00323-15

FIG 2.

FIG 2

Purification of Est_1092 from L. plantarum DSM 1055. SDS-PAGE analysis of the expression and purification of Est_1092. Soluble cell extracts of IPTG-induced E. coli BL21(DE3)(pURI3-Cter) (lane 1) or E. coli BL21(DE3)(pURI3-Cter-1092) (pGro7) (lane 2), flowthrough from the affinity resin (lane 3), and protein eluted after His affinity resin (lane 4) were analyzed. Arrow, the overproduced and purified protein. The 12.5% gel was stained with Coomassie blue. Molecular mass markers are located in the left lane (SDS-PAGE standards; Bio-Rad).