TABLE 3.
Novel and putatively novel secondary metabolites from the selected isolates
| Genus and strain | Fermentation medium | Compound(s) produced | Structural class | Chemical data |
|
|---|---|---|---|---|---|
| UV (MeOH) | MS (m/z)c | ||||
| Streptomycetes | |||||
| Streptomyces sp. FXJ1.030 | GYM | 1 putative novel compound | —g | 265, 492 | [M-H]− = 1,034.7 |
| Streptomyces sp. FXJ1.050a | SGG | 2 putative novel compounds, related to niphimycins | Macrolide | 225, 231 (sh) | [M-H]− = 1,140.7159; [M+H]+ = 1,142.7312 |
| Streptomyces sp. FXJ1.066 | SGG | 1 putative novel compound | Peptide-type compound | 221, 280 | [M-H]− = 1,022.7 |
| SGG | 5 new products related to spoxazomicins | Siderophore | 243, 302 | [M+H]+ = 775.2, 757.2, 818.3, 789.2, 803.3 | |
| Streptomyces sp. FXJ1.068 | GYM | 1 new analogue of nanchangmycind | Polyether | 234 | [M+Na]+ = 903.7 |
| Streptomyces sp. FXJ1.069a,b | GYM | 5 putative novel compounds | — | 249, 289/295 | [M+Na]+ = 609.6, 609.6 |
| — | 249, 366 | [M+H]+ = 595.5 | |||
| — | 240, 319, 721/731 | [M+H]+ = 333.1, 333.2 | |||
| SGG | 2 putative novel compounds | — | 249 | [M-H]− = 275.1647 | |
| — | 238, 262 | MS no ion current | |||
| Streptomyces sp. FXJ1.075a | MOSY | 3 putative novel compounds | — | 257, 369 | [M+H]+ = 371.4 |
| — | 228 | [M+H]+ = 761.9 | |||
| — | 220, 271, 290 | [M+H]+ = 1,058.9 | |||
| Streptomyces sp. FXJ1.076a | GYM/MOSY | 2 elaiophylin-type new compoundse | Macrolide | 250 | [M+HCOO]− = 759.4320 |
| 249 | [M+HCOO]− = 921.5212 | ||||
| Streptomyces sp. FXJ1.172a | MOSY | 1 new analogue of thienodolind | — | 239, 292, 334 | [M-H]− = 264.9839 |
| GYM | 6 putative novel compounds | — | End absorbance | [M-H]− = 545.5 | |
| — | 230, 297 | [M+H]+ = 526.3427, 540.3597, 554.3679, 554.3745, 568.3896 | |||
| SGG | 6 putative novel compounds | Peptide-type compound | 277/279/277/281/276/277 | [M+H]+ = 742.3423, 798.4035, 812.4167, 826.4343, 840.4512, 920.4258 | |
| Streptomyces sp. FXJ1.234 | SGG | 4 putative novel compounds | — | 253, 291 | [M+Na]+ = 919.4, 1065.6, 1,079.6, 1,051.5 |
| Streptomyces sp. FXJ1.235b | MOSY/SGG | 6 putative novel compounds | Chlorinated compounds | 244, 256, 265, 294,307, 340 | [M+H]+ = 304.0403, 445.0760 |
| — | 243, 256, 265, 305, 332 | [M+H]+ = 270.0809 | |||
| — | 244, 256, 265, 294,307, 338 | [M+H]+ = 437.2 | |||
| — | 244, 256, 265, 294,307, 340 | 2 MS no ion current | |||
| Streptomyces sp. FXJ1.250b | GYM | 1 putative novel compounds | — | 295 | [M-H]− = 407.5 |
| Streptomyces sp. FXJ1.253a | MOSY | 3 putative novel compounds | — | 249/249/269, 253 | MS no ion current |
| Streptomyces sp. FXJ1.264 | GYM | 1 new analogue of etheromycine | Polyether | None | [M-H]− = 927.5659 |
| GYM | 8 putative novel compounds related to oxazolomycins | PKS-NRPS hybridf | 276, 334/334/336/326/327/326/331/365 | [M+H]+ = 568.4, 568.3, 568.3, 452.3, 452.2, 480.2, 480.3, 665.3 | |
| GYM/MOSY | 4 putative novel compounds | Related to siderophore | 270 | [M+H]+ = 670.2627, 670.2666, 684.2830, 684.2815 | |
| Streptomyces sp. FXJ1.275a,b | MOSY | 1 putative novel quinomycin-type compound | Peptide-type compound | 246, 330 | [M+H]+ = 1,151.6 |
| SGG | 2 putative novel compounds | — | 236, 353/236, 354 | MS no ion current | |
| Streptomyces sp. FXJ1.310 | GYM | 1 putative novel compound | — | 264, 493 | [M-H]− = 559.5 |
| Streptomyces sp. FXJ1.408a,b | GYM/MOSY/SGG | 4 brunefungin- or flavopentin-type new compounds | Polyene macrolide | 264, 363 | [M+H]+ = 735.2, the other 3 MS not measured |
| Streptomyces sp. FXJ1.409 | MOSY | 2 putative novel compounds | Related to peptide | 218 (sh), 280, 316 (sh)/325 (sh) | [M+H]+ = 842.2603, 617.1464 |
| Streptomyces sp. FXJ1.414a,b | GYM/MOSY/SGG | 4 brunefungin- or flavopentin-type new compounds | Polyene macrolide | 264, 363 | [M+H]+ = 735.2, the other 3 MS not measured |
| Streptomyces sp. FXJ1.532a,b | GYM | 2 new macrolide compoundse | Macrolide | 229 | [M+Na]+ = 789.4402, 789.4402 |
| Streptomyces sp. FXJ1.535a,b | SGG | 1 putative novel compounds | Angucycline-type compound | 259, 398 | [M-H]− = 413.2692 |
| Streptomyces sp. GTVL2G15b | MOSY | 7 putative novel compounds | — | 250, 282, 364 | [M+H]+ = 295.9, 294.0 |
| — | 252, 270 (sh), 474 | [M-H]− = 459.2 | |||
| — | 291 | MS no ion current | |||
| Actinomycin-like compound | 231, 420, 441 | [M/2+H]+ = 628.3 | |||
| — | 229, 272, 344, 409 | MS no ion current | |||
| — | 228, 275, 338, 432 | [M-H]− = 339.2 | |||
| SGG | 1 Oxachelin analogue | Siderophore | 242, 250, 260 (sh), 303 | Peak degraded before MS | |
| Streptomyces sp. FXJ23ya,b | GYM/SGG | 3 putative novel compounds | — | 243, 366 | MS no ion current |
| — | 211 | [M-H]− = 649.8, 649.8 | |||
| Nonstreptomycete actinomycetes | |||||
| Saccharothrix sp. FXJ1.021a | MOSY | 6 putative novel compounds | Related to anthracycline | 253, 362 | [M+H]+ = 323.0798 |
| 262, 377 | [M+H]+ = 505.2234 | ||||
| 254, 400 | [M+H]+ = 489.2273 | ||||
| 254, 370 | [M+H]+ = 505.2212 | ||||
| 258, 373 | [M+H]+ = 505.2193 | ||||
| 276, 370 | [M+H]+ = 489.2285 | ||||
| Lentzea jiangxiensis FXJ1.034Ta,b | SGG | 4 putative novel compounds | 3 related to siderophore | 285 | [M+Na]+ = 669.2 |
| 205, 245, 312 | [M+H]+ = 654.4 | ||||
| 206, 243, 311 | 2 MS no ion current | ||||
| Nonomuraea jiangxiensis FXJ1.102Ta,b | SGG | 1 putative novel compound related to antibiotic BE 14106 | Macrolactam | 260, 270, 310 (sh) | [M+H]+ = 474.5 |
| SGG | 3 putative novel compounds | — | 236, 289, 318 | [M+H]+ = 972.9 | |
| — | 236, 289, 318 | 2 MS no ion current | |||
| Amycolatopsis sp. FXJ1.244a,b | GYM/MOSY/SGG | 5 new peptides | Peptide | 212 | [M+H]+ = 1,195.6, 1,181.7, 1,181.6, 935.7, 100.2 |
| Actinomadura sp. FXJ1.344a | SGG | 3 homoprejadomycin-type new derivatives | Angucycline | 266, 385/397/401 | [M+H]+ = 659.6, 323.3, 759.1 |
| 1 putative novel compound | — | 225, 314, 429 | [M+H]+ = 637.6 | ||
| Amycolatopsis sp. FXJ1.406a | GYM/MOSY/SGG | 5 new peptides | Peptide | 212 | Similar to those of FXJ1.244 |
| Amycolatopsis sp. FXJ1.428a | SGG | 1 putative novel compound | — | 214, 285 | [M+H]+ = 637.4 |
| Amycolatopsis sp. FXJ1.444a | SGG | 1 putative novel compound | — | 214, 285 | [M+H]+ = 637.4 |
| Sphaerisporangium sp. FXJ1.452a | SGG | 2 putative novel compound | — | 225, 236, 260 (sh), 335 | [M+H]+ = 897.4116, 883.3928 |
| Pseudonocardia sp. FXJ1.453a | SGG | 1 putative novel compound | — | 250, 310 | [M+H]+ = 406.1264 |
| Microbispora sp. FXJ1.457a,b | MOSY | 5 putative novel compounds | — | 262–263, 362–363 | 5 MS no ion current |
| Streptosporangium sp. FXJ1.481a | SGG | 1 putative novel compound identical with that from FXJ1.544 | — | 214, 247 | [M+H]+ = 375.1448 |
| Actinoallomurus sp. FXJ1.503a | SGG | 1 putative novel compound | — | 257 | [M+NH4]+ = 507.3441 |
| Actinocorallia sp. FXJ1.544 | SGG | 1 putative novel compound, identical with that from FXJ1.481 | — | 214, 247 | [M+H]+ = 375.1442 |
| Actinomadura sp. FXJ1.848a | MOSY | 1 putative novel compound | — | 234, 256 (sh), 280 (sh), 308, 320 (sh) | [M+H]+ = 488.2 |
| Microbispora sp. FXJ1.1062b | SGG | 2 putative novel compounds | — | 246 | [M+H]+ = 466.3160, 403.2486 |
| Streptosporangium sp. FXJ1.1111a | SGG | 4 putative novel compounds | Siderophore | 247, 314 | [M+H]+ = 467.2036, 425.1935, 466.2185, 449.1921 |
a
Strain is assigned to the acidophilic group.
b
Strain represents a putative new species; FXJ1.034T and FXJ1.102T have been proposed as type strains of Lentzea jiangxiensis sp. nov. and Nonomuraea jiangxiensis sp. nov., respectively (46, 47).
c
Values accurate to 1 decimal place were generated by regular MS, and values accurate to 4 decimal places were generated by HR-MS.
d
Compound was subjected to tandem MS analysis.
e
Compound was subjected to NMR analysis.
f
PKS-NRPS, polyketide synthase-nonribosomal peptide synthetase.
g
—, compounds not classified.