FIG 3.
Analysis of Erm(44)-RGS-His6 protein synthesis in S. aureus RN4220 (A) and E. coli AG100A or AG100 (B) cells harboring the expression vector for erm(44) or erm(44)-rgs-his6. Thirty micrograms of total protein extract per sample was separated using 15% SDS-PAGE and analyzed through Western blotting. The upper part of the membrane was probed with anti-RGS-His antibody (5 Prime GmbH, Hilden, Germany) to detect tagged Erm(44), and the lower part of the membrane was probed with antiserum specific for the E. coli ribosomal protein L20ΔN (41), which served as a loading control. The asterisk at 15 kDa indicates the weak possible interaction of the antibody specific to E. coli L20ΔN with an S. aureus homolog. Prestained peqGOLD protein marker IV molecular size markers were used.