Strains |
|
|
S. aureus
|
|
|
RN4220 |
NCTC8325-4 derivative, antibiotic susceptible, restriction deficient; sau1 hsdR (rK− mK+) |
49 |
RN4220/pVPF5 |
RN4220 containing plasmid pVPF5 from S. xylosus VF5 |
43 |
E. coli
|
|
|
DH5α |
K-12 strain; recA1 endA1 hsdR17 (rK− mK+) |
Life Technologies |
AG100 |
K-12 strain |
50 |
AG100A |
AG100 ΔacrAB::aphA1 (Kmr) mutant; increased susceptibility to antibiotics |
44 |
B. subtilis
|
|
|
DSM4514 |
Derivative of strain 168 containing plasmid pUB110 |
DSMZ |
Plasmids |
|
|
pUB110 |
S. aureus plasmid, replicates in B. subtilis; aadD (Kmr Neor) |
51 |
pVPF5 |
S. xylosus pT181 family plasmid; tet(K) |
43 |
pBUS1 |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, ColE1, MCS pBluescript II SK (Stratagene), (rrnB T1)5
tet(L) |
24 |
pBUS1-Pcap
|
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, ColE1, promoter of S. aureus type 1 capsule gene 1A (Pcap) upstream of a new MCS with joinable rgs-his6 codons (Pcap-MCS-rgs-his6), (rrnB T1)5
tet(L) |
This study |
pBUS1-HC |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, sso oriL ColE1, MCS pBluescript II SK (Stratagene), (rrnB T1)5
tet(L) |
This study |
pBUS1-Pcap-HC |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, sso oriL ColE1 Pcap-MCS-rgs-his6 (rrnB T1)5
tet(L) |
This study |
pTSSC |
S. aureus-E. coli shuttle vector; pT181 replicon, ColE1, MCS pBluescript II SK (Stratagene), (rrnB T1)5
tet(L) |
This study |
pTSSC-Pcap
|
S. aureus-E. coli shuttle vector; pT181 replicon, ColE1 Pcap-MCS-rgs-his6 (rrnB T1)5
tet(L) |
This study |
pTSSCm |
pTSSC with two silent mutations in the repC sequence to remove HindIII and XbaI sites |
This study |
pTSSCm-Pcap
|
pTSSC-Pcap with two silent mutations in the repC sequence to remove HindIII and XbaI sites |
This study |
pBJW13 |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon; ColE1 Pcap erm(44) (MLSBr) (rrnB T1)5
tet(L) |
26 |
pBJW13-HC |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, sso oriL ColE1 Pcap erm(44) (rrnB T1)5
tet(L) |
This study |
pTJW13 |
S. aureus-E. coli shuttle vector; pT181 replicon, ColE1 Pcap erm(44) (rrnB T1)5
tet(L) |
This study |
pBJW13-RGS-His |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, ColE1 Pcap erm(44)-rgs-his6 (rrnB T1)5
tet(L) |
This study |
pBJW13-HC-RGS-His |
S. aureus-E. coli shuttle vector; pAMα1 minimum replicon, sso oriL ColE1 Pcap erm(44)-rgs-his6 (rrnB T1)5
tet(L) |
This study |
pTJW13-RGS-His |
S. aureus-E. coli shuttle vector; pT181 replicon, ColE1 Pcap erm(44)-rgs-his6 (rrnB T1)5
tet(L) |
This study |