TABLE 3.
Estimated plasmid copy numbers of S. aureus RN4220 transformants determined by quantitative real-time PCR
| Plasmid |
CT (avg ± SD)a |
Avg no. of copies (CV [%])b |
PCN (avg ± SD)c | ||
|---|---|---|---|---|---|
| nuc | tet(L) | nuc | tet(L) | ||
| None | 21.44 ± 0.03 | 3.12 × 105 (1.8) | |||
| pBUS1 | 21.52 ± 0.06 | 20.59 ± 0.11 | 2.97 × 105 (3.4) | 3.71 × 106 (6.9) | 12.48 ± 0.86 |
| pBUS1-Pcap | 22.04 ± 0.09 | 20.48 ± 0.10 | 2.16 × 105 (5.4) | 3.98 × 106 (6.4) | 18.45 ± 1.18 |
| pBUS1-HC | 21.56 ± 0.02 | 20.15 ± 0.03 | 2.91 × 105 (1.4) | 4.87 × 106 (1.9) | 16.74 ± 0.31 |
| pBUS1-Pcap-HC | 21.28 ± 0.05 | 19.55 ± 0.07 | 3.45 × 105 (3.3) | 7.12 × 106 (4.2) | 20.60 ± 0.86 |
| pTSSC | 21.00 ± 0.05 | 19.20 ± 0.02 | 4.12 × 105 (2.8) | 8.81 × 106 (1.5) | 21.41 ± 0.32 |
| pTSSC-Pcap | 20.77 ± 0.06 | 18.55 ± 0.10 | 4.73 × 105 (3.9) | 1.32 × 107 (6.1) | 27.93 ± 1.71 |
| pTSSCm | 20.98 ± 0.02 | 19.18 ± 0.03 | 4.17 × 105 (1.3) | 8.91 × 106 (2.1) | 21.39 ± 0.44 |
| pTSSCm-Pcap | 21.25 ± 0.02 | 19.06 ± 0.06 | 3.52 × 105 (1.2) | 9.59 × 106 (4.0) | 27.27 ± 1.06 |
a
n = 3.
b
CV, coefficient of variation (n = 3).
c
PCN, plasmid copy number (n = 3).