FIG 2.

Map of the hin-iroB intergenic sequence of various monophasic variants. The intergenic sequence lying between the hin and iroB genes and observed in the Salmonella Typhimurium LT2 reference strain as well as in some representative isolates of the monophasic variants described in this work is shown as a black box. IS26 elements are indicated by hatched arrows. Nucleotide positions in the intergenic sequence bordering IS26 elements are given in white boxes above vertical arrows and follow the nucleotide sequence numbering of Salmonella Typhimurium LT2 (AE006468). Compared to S. Typhimurium LT2 (panel a), the hin-iroB intergenic sequence was deleted by 536 (panel b), 482 (panel c), or 451 (panel d) bp. LJ and RJ indicate the left and right junctions, respectively, amplified by the junction PCRs described in Materials and Methods. The dotted line in panel d delineates the large (56.6-kb) intervening region detailed in Fig. 1.