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Schematic representation of plasmid vector pMaORI. Plasmid pMaORI (5,035 bp) was generated by cloning the replication origin of L. mayottensis strain 200901116, which carries the rep, parA, and parB genes, into the XbaI-BamHI restriction sites of conjugative plasmid pMAT, which contains the spectinomycin resistance cassette (aadA/Spcr), ori R6K, and oriT RK2/RP4. The aadA gene is expressed from the Borrelia burgdorferi flgB promoter.
