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. 2015 Apr 10;81(9):3029–3038. doi: 10.1128/AEM.04269-14

FIG 6.

FIG 6

Site-directed mutagenesis of PhaZh1. (A) SDS-PAGE analysis of PhaZh1 muteins purified from the haloarchaeon H. volcanii H1424 harboring pTA03 plasmid derivatives of phaZh1 mutants. Lanes D195A, S47A, and G16A, Asp195, Ser47, and Gly16 in the amino acid sequence of PhaZh1 were replaced with alanine; lane WT, wild-type PhaZh1; lane M, protein markers. (B) Turbidimetric assay (OD650) of the hydrolysis of nPHA granules (isolated from the haloarchaeon H. mediterranei EPSΔphaZh1) by purified PhaZh1 and its muteins. Control, without purified protein.