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. Author manuscript; available in PMC: 2016 Apr 9.
Published in final edited form as: Cell. 2015 Apr 9;161(2):255–263. doi: 10.1016/j.cell.2015.02.042

Figure 2. Steamer retrotransposon DNA copy numbers in different clam tissues.

Figure 2

Quantitative PCR was used to determine the copy number of the Steamer retrotransposon in genomic DNA from hemocytes and solid tissues of four representative normal (left) and four leukemic (right) clams. Steamer copy number was quantitated using primers that amplify a region in the Steamer reverse transcriptase (RT) and was normalized to the single-copy gene EF1 (Siah et al., 2011). * indicates P < 0.01 for comparisons between neoplastic hemocytes and each other tissue tested (n = 3 for gills, n = 4 for other tissues), using two-tailed paired T-test of normalized Steamer copy number.