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. Author manuscript; available in PMC: 2016 Apr 9.
Published in final edited form as: Cell. 2015 Apr 9;161(2):348–360. doi: 10.1016/j.cell.2015.02.044

Figure 3. BpBimA and BmBimA oligomerization is required for actin nucleation and barbed end binding.

Figure 3

(A) Alignment of the trimeric coiled coils from BpBimA and BmBimA with the Hia trimeric coiled coil (Meng et al., 2006). Positions replaced with Asp residues in the BimA8D mutants are outlined in black. Hydrophobic residues are blue and charged residues orange. (B) Domain schematics of wild-type and BimA8D mutants in which eight positions were changed to Asp residues are shown above gel filtration elution profiles of wild-type and mutant proteins. (C) The time to half-maximum fluorescence normalized to actin alone in polymerization reactions with increasing BimA8D proteins. The means ± SD are shown with the wild-type data from Figure 1C for reference. (D) Elongation reactions with 10 nM CapZ and with our without BimA8D proteins.