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. Author manuscript; available in PMC: 2016 Apr 9.
Published in final edited form as: Cell. 2015 Apr 9;161(2):277–290. doi: 10.1016/j.cell.2015.02.016

Figure 3. Identification of macro-environmental modulators following hair plucking.

Figure 3

(A) TUNEL assay to measure apoptosis. (B) Real time PCR from extra-follicular macro-environmental tissues revealed the kinetics of gene expression induced by plucking (Normalized to GAPDH with 40 cycles, data are represented as mean +/− S.D., n=3).

(C) Whole mount in situ hybridization showed that Tnf-α is markedly up-regulated in the inter-follicular area beginning 2 days after wax stripping. See also Figure S3, S4.