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. 2015 Apr 8;11:86. doi: 10.1186/s12917-015-0398-5

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© Zhang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot for Col X from cultured chondrocytes exposed by different dose of CORT. (A) Immunoreactive bands for Col X and GAPDH protein. (B) Statistical analysis of Col X. Cultures were lysed and protein was extracted, quantitated and analyzed via Western blot analysis. Representative blot for each group from triplicate experiments was shown. Col X protein band density was quantitated by densitometry using the Quantity One software. Col X protein level was expressed as the Col X: Gapdh ratio. *P < 0.05 and **P < 0.01 versus control (0 M CORT).