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. 2015 Mar 12;13:12. doi: 10.1186/s12953-015-0066-5

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© Zhang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Workflow of the iTRAQ proteomic strategy. In this work, three pathologically verified tissue samples of PsPD and three samples of GBM were used for iTRAQ labeled proteomic analysis. The proteins identified were quantitatively analyzed using Panther and IPA for biological functions analysis. Several candidate proteins with interesting biological functions were selected and further validated using IHC and WB of the same samples used for proteomic analysis as well as additional samples.