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. 2015 Mar 18;15:140. doi: 10.1186/s12885-015-1135-y

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© Kachel et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evaluation of total- and Phospho-LDHA protein expression. Total and y10-phosphorylated LDHA expressions in goiter, FA, FTC, PTC and UTC were analysed by employment of western blot analysis and evaluated densitometrically with ImageJ software. Protein expression was measured in relation to FTC133 as positive control and then normalized to the expression of ß-actin as normalizing marker. Differences are expressed as percent to positive control defined as 100%. *p < 0.05 indicates a statistical significance.