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. 2015 Mar 11;6(1):17. doi: 10.1186/s13287-015-0008-2

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© Li et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Quantitative real-time-polymerase chain reaction and Western blot analysis of keratinocyte markers after a 21-day treatment with two different keratinocyte-conditioned media. (A) mRNA expression of cytokeratin-5, cytokeratin-14, stratifin, involucrin, and filaggrin in bone marrow mesenchymal stem cells (BMSCs), keratinocytes, and KCM1 and KCM2 groups. Keratinocytes at passages 2 to 5 and BMSCs at passages 4 to 7 were used as positive and negative controls, respectively. Keratinocytes were normalised to 1 because these cells expressed all of the markers. (B) Western blot analysis of cytokeratin-5, cytokeratin-14, stratifin, involucrin, and filaggrin in BMSCs, keratinocytes, and KCM1 and KCM2 groups. Keratinocytes at passages 2 to 5 and BMSCs at passages 4 to 7 were used as positive and negative controls, respectively. Beta-actin was used as a loading control. Data are presented as the mean ± standard error of the mean. n = 3. *P <0.05, **P <0.01 BMSCs versus KCM1/KCM2, ^# P <0.05, ^## P <0.01 KCM1 versus KCM2. CK-5, cytokeratin-5; CK-14, cytokeratin-14; KCM1, keratinocyte-conditioned medium collected from keratinocytes with no Y-27632 treatment; KCM2, keratinocyte-conditioned medium collected from keratinocytes treated with Y-27632.