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The oligonucleotid primers and probes used in real time PCR assay
| Target and internal (bp) control genes | Sequence | Amplicon size |
|---|---|---|
| YBX2 | F: CCCTACCCAGTACCCTGCT | 150 |
| R: CCTTCCTTCAACCCTTGATAA | ||
| P: CAGGAGGACCAAAGCAGCAGCC | ||
| JHDM2A | F: GTTCCACAAGCATTGACTGG | 145 |
| R: CTGGTGCATTTGAAACATCC | ||
| P: TGCCAATCCTCCTGAACTGCAGA | ||
| GAPDH | F: TCAAGAAGGTGGTGAAGCAG | 93 |
| R: CGCTGTTGAAGTCAGAGGAG | ||
| P: CCTCAAGGGCATCCTGGGCT | ||
Optimization of real-time PCR assay
