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. 2015 Apr 8;17(1):71–82. doi: 10.22074/cellj.2015.513

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Fig.2 — Real-time RT-PCR for relative gene expression of (A) RUNX2, (B) OSX, (C) DLX5 and (D) BSP during osteoblastic differentiation of MSCs.

Day 0; Undifferentiated MSCs, Days 7, 14 and 21; Osteoblastic differentiated cells days 7, 14, 21, respectively. Gene expression of RUNX2 and OSX increases slightly whereas gene expression of DLX5 and especially BSP increases markedly during osteoblastic differentiation of MSCs. Triplicates were made for each approach.

RT-PCR; Reverse transcription polymerase chain reaction, MSCs; Mesenchymal stem cells, RUNX2; Runt-related transcription factor 2, OSX; Osterix, DLX; Distal-less homeobox 5 and BSP; Bone sialoprotein.