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. 2015 Apr 8;17(1):71–82. doi: 10.22074/cellj.2015.513

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Fig.3 — MSP results with methylated (M) and unmethylated (U) primers for (A) RUNX2 and (B) DLX5. Day 0; Undifferentiated MSCs, Days 7, 14 and 21; Osteoblastic differentiated cells days 7, 14, 21, respectively, P; Positive control (methylated and SBS treated DNA in M experiments and only SBS treated DNA in U experiments), U; SBS untreated DNA as negative control for MSP and Neg; No DNA as negative control. Amplicon size in RUNX2: mexperiment (93 bp) and U experiment (96 bp). Amplicon size in DLX5: M experiment (150 bp) and U experiment (147 bp).

MSP; Methylation-specific PCR, RUNX2; Runt-related transcription factor 2, DLX; Distal-less homeobox 5, MSCs; Mesenchymal stem cells, SBS; Sodium bisulfite.