Table 1.
Primers of tetra ARMS for 9p21 locus polymorphisms including rs1333049 and rs10757274
| Type | Rs1333049 polymorphismSequence | CG% | Tm |
|---|---|---|---|
| OF primer | 5´-CGAAGTAGAGCTGCAAAGATATTTGGAA-3´ | 39.3 | 62.2 |
| OR primer | 5´-GGGCTCATAATTGCTGAATAAAACAGAA-3´ | 35.7 | 60.7 |
| IF primer | 5´-CCTCATACTAACCATATGATCAACAGATG-3´ | 37.9 | 62.4 |
| IR primer | 5´-CTTACCTCTGCGAGTGGCTGCTTATG-3´ | 53.8 | 66.4 |
| rs10757274 polymorphism | |||
| OF primer | 5´-TATGTAATGGCCTTCTTTGTCTCTTTTG-3´ | 35.7 | 60.7 |
| OR primer | 5´-AATGAATAAATGCTAACTTCTGCCTCAC -3´ | 35.7 | 60.7 |
| IF primer | 5´-GTGGGTCAAATCTAAGCTGAGTGTGGA -3´ | 48.1 | 64.9 |
| IR primer | 5´-ATCTATCTAGTGAATTTCAATTATGGCC -3´ | 32.1 | 59.2 |
ARMS; Amplification refractory mutation system, Tm; Melting temperature, OF; Outer forward, IF; Inner forward, OR: Outer reverse and IR; Inner reverse.