Table 2.

The PCR thermal cyclers of the rs1333049 polymorphism

Stage	Number of repeat	Section	Temperature (˚C)	Time
First	1 cycle	First denaturation	95	5 minutes
		Secondary denaturation	95	45 seconds
Second	30 cycles	Annealing	62	90 seconds
		First extension	72	70 seconds
Third	1 cycle	Secondary extension	72	7 minutes

PCR; Polymerase chain reaction.