Table 3.
Stage | Number of repeat | Section | Temperature (˚C) | Time |
---|---|---|---|---|
First | 1 cycle | First denaturation | 95 | 5 minutes |
Secondary denaturation | 95 | 45 seconds | ||
Second | 35 cycles | Annealing | 52 | 90 seconds |
First extension | 72 | 90 seconds | ||
Third | 1 cycle | Secondary extension | 72 | 7 minutes |
PCR; Polymerase chain reaction.