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. 2015 Apr 8;17(1):89–98. doi: 10.22074/cellj.2015.515

Table 3.

The PCR thermal cyclers of the rs10757274


Stage Number of repeat Section Temperature (˚C) Time

First 1 cycle First denaturation 95 5 minutes
Secondary denaturation 95 45 seconds
Second 35 cycles Annealing 52 90 seconds
First extension 72 90 seconds
Third 1 cycle Secondary extension 72 7 minutes

PCR; Polymerase chain reaction.