Fig 4.

Effects of isoform-selective inhibitors of rho-associated kinase (ROK) 1 and ROK 2 on resting and stimulated phosphorylation of regulatory light chain (RLC) in hUM. Both the ROK 1-selective inhibitor (Rho-15) (A) and the ROK 2-selective inhibitor (SR3677) (B) suppressed basal pRLC and ppRLC in hUM (n = 4; P < 0.01 by one-way anova), similarly to the less selective inhibitor g-H as noted in Figure2A. Neither Rho-15 (25 μM) (C) nor SR3677 (25 μM) (D) affected the OT-induced responses in pRLC, but both abolished the responses in ppRLC (n = 4; P < 0.01 by two-way anova), in a pattern identical to g-H (Fig.3).