Fig 6.

Effects of activation of rhoA on phosphorylation of regulatory light chain (RLC) in hUM. (A) Activation of rhoA, assessed by measuring GTP-bound rhoA, was significantly increased by OT (100 nM) and by the rhoA activator Calpeptin (Calp; 0.5 units/ml). These data have been normalized to rhoA peptide content assessed using Western blot. (n = 4; histograms with different superscripts are significantly different from each other using one-way anova and post hoc Tukey test). (B) Treatment with Calp caused an increase in pRLC that was not significantly affected by inhibition of MLCK using ML7 (25 μM) or inhibition of ROK using g-H (1 μM). (C) In contrast, the Calp-stimulated responses in ppRLC were significantly decreased with either ML7 or g-H (1 μM) (n = 4–8; P < 0.01 by two-way anova).